Antigen

Antigen Class

TFs and others

Antigen

RAD21

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7 cells

cell line

MFC7

cell type

Breast adenocarcinoma

chip antibody

RAD21, abcam, ab992

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cross-linked chromatin was immunoprecipitated with an antibody. The Protein:DNA crosslinks were then reversed and the DNA fragments were recovered and sequenced. Please see protocol notes below and go to http://hudsonalpha.org/myers-lab/protocols for the most current version of the protocol. Biological replicates from each experiment were completed. For RNA-seq polyA RNA was isolated using oligo dT beads, and libraries prepared. Illumina Single End

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

32944013

Reads aligned (%)

95.5

Duplicates removed (%)

4.3

Number of peaks

35073 (qval < 1E-05)

hg19

Number of total reads

32944013

Reads aligned (%)

94.9

Duplicates removed (%)

5.4

Number of peaks

35953 (qval < 1E-05)